{"id":238,"date":"2025-06-16T16:23:09","date_gmt":"2025-06-16T16:23:09","guid":{"rendered":"https:\/\/radweb.su.domains\/cirp\/?page_id=238"},"modified":"2025-06-16T16:33:00","modified_gmt":"2025-06-16T16:33:00","slug":"mri-protocol","status":"publish","type":"page","link":"https:\/\/radweb.su.domains\/cirp\/resources\/mri-protocol\/","title":{"rendered":"MRI Protocol"},"content":{"rendered":"\t\t<div data-elementor-type=\"wp-page\" data-elementor-id=\"238\" class=\"elementor elementor-238\">\n\t\t\t\t\t\t<section class=\"elementor-section elementor-top-section elementor-element elementor-element-49121c1 elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"49121c1\" data-element_type=\"section\" data-e-type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-100 elementor-top-column elementor-element elementor-element-3bfa08f\" data-id=\"3bfa08f\" data-element_type=\"column\" data-e-type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t<div class=\"elementor-element elementor-element-ff65ed1 elementor-widget elementor-widget-text-editor\" data-id=\"ff65ed1\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<h3><strong>MR Imaging of Murine Osteosarcomas<\/strong><\/h3><p>We use <strong>magnetic resonance imaging (MRI)<\/strong> to investigate osteosarcomas in mouse legs. Scans are performed on an actively-shielded Bruker 7T horizontal bore scanner and include pre-treatment, post-treatment, and enhanced post-contrast scans 24 hours after intravenous infusion of 30 mg Fe\/kg body weight of iron oxide nanoparticles. To confirm nanoparticle co-localization with immune cells in the tumor microenvironment, we can conjugate the iron oxide nanoparticles to FITC or rhodamine before administration.<\/p><p>Our MRI protocol uses the following pulse sequences:<\/p><ul><li><strong>T2-weighted fast spin echo (FSE)<\/strong> for anatomical reference (TR: 2500 ms, TE: 33 ms).<\/li><li><strong>Multi-gradient echo (MGE)<\/strong> for T2*-mapping (Flip angle: 50 degrees, TR: 800 ms, minimum TE: 3.5 ms, echo spacing: 5 ms, ETL: 10).<\/li><\/ul><p>Both sequences use a 2.56 cm \u00d7 2.56 cm field of view (FOV) and a 256 x 256 pixel image matrix.<\/p><p><strong>T2*-maps<\/strong> are calculated from the MGE image series using a mono-exponential decay model with a constant offset:<\/p><p>S(t)=A+S0\u200b\u2217exp(\u2212t\/T)<\/p><p>Where:<\/p><ul><li>S(t) is the signal at time t<\/li><li>A is the absolute bias<\/li><li>S0\u200b is the signal intensity<\/li><li>T is T2*<\/li><\/ul><p>These parameters are then evaluated and mapped using Paravision 7.1.<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<\/div>\n\t\t","protected":false},"excerpt":{"rendered":"<p>MR Imaging of Murine Osteosarcomas We use magnetic resonance imaging (MRI) to investigate osteosarcomas in mouse legs. Scans are performed on an actively-shielded Bruker 7T horizontal bore scanner and include pre-treatment, post-treatment, and enhanced post-contrast scans 24 hours after intravenous infusion of 30 mg Fe\/kg body weight of iron oxide nanoparticles. To confirm nanoparticle co-localization [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"parent":21,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_monsterinsights_skip_tracking":false,"ocean_front_end_style_editor":"no","ocean_post_layout":"","ocean_both_sidebars_style":"","ocean_both_sidebars_content_width":0,"ocean_both_sidebars_sidebars_width":0,"ocean_sidebar":"","ocean_second_sidebar":"","ocean_disable_margins":"enable","ocean_add_body_class":"","ocean_shortcode_before_top_bar":"","ocean_shortcode_after_top_bar":"","ocean_shortcode_before_header":"","ocean_shortcode_after_header":"","ocean_has_shortcode":"","ocean_shortcode_after_title":"","ocean_shortcode_before_footer_widgets":"","ocean_shortcode_after_footer_widgets":"","ocean_shortcode_before_footer_bottom":"","ocean_shortcode_after_footer_bottom":"","ocean_display_top_bar":"default","ocean_display_header":"default","ocean_header_style":"","ocean_center_header_left_menu":"","ocean_custom_header_template":"","ocean_custom_logo":0,"ocean_custom_retina_logo":0,"ocean_custom_logo_max_width":0,"ocean_custom_logo_tablet_max_width":0,"ocean_custom_logo_mobile_max_width":0,"ocean_custom_logo_max_height":0,"ocean_custom_logo_tablet_max_height":0,"ocean_custom_logo_mobile_max_height":0,"ocean_header_custom_menu":"","ocean_menu_typo_font_family":"","ocean_menu_typo_font_subset":"","ocean_menu_typo_font_size":0,"ocean_menu_typo_font_size_tablet":0,"ocean_menu_typo_font_size_mobile":0,"ocean_menu_typo_font_size_unit":"px","ocean_menu_typo_font_weight":"","ocean_menu_typo_font_weight_tablet":"","ocean_menu_typo_font_weight_mobile":"","ocean_menu_typo_transform":"","ocean_menu_typo_transform_tablet":"","ocean_menu_typo_transform_mobile":"","ocean_menu_typo_line_height":0,"ocean_menu_typo_line_height_tablet":0,"ocean_menu_typo_line_height_mobile":0,"ocean_menu_typo_line_height_unit":"","ocean_menu_typo_spacing":0,"ocean_menu_typo_spacing_tablet":0,"ocean_menu_typo_spacing_mobile":0,"ocean_menu_typo_spacing_unit":"","ocean_menu_link_color":"","ocean_menu_link_color_hover":"","ocean_menu_link_color_active":"","ocean_menu_link_background":"","ocean_menu_link_hover_background":"","ocean_menu_link_active_background":"","ocean_menu_social_links_bg":"","ocean_menu_social_hover_links_bg":"","ocean_menu_social_links_color":"","ocean_menu_social_hover_links_color":"","ocean_disable_title":"default","ocean_disable_heading":"default","ocean_post_title":"","ocean_post_subheading":"","ocean_post_title_style":"","ocean_post_title_background_color":"","ocean_post_title_background":0,"ocean_post_title_bg_image_position":"","ocean_post_title_bg_image_attachment":"","ocean_post_title_bg_image_repeat":"","ocean_post_title_bg_image_size":"","ocean_post_title_height":0,"ocean_post_title_bg_overlay":0.5,"ocean_post_title_bg_overlay_color":"","ocean_disable_breadcrumbs":"default","ocean_breadcrumbs_color":"","ocean_breadcrumbs_separator_color":"","ocean_breadcrumbs_links_color":"","ocean_breadcrumbs_links_hover_color":"","ocean_display_footer_widgets":"default","ocean_display_footer_bottom":"default","ocean_custom_footer_template":"","footnotes":""},"class_list":["post-238","page","type-page","status-publish","hentry","entry"],"aioseo_notices":[],"aioseo_head":"\n\t\t<!-- All in One SEO 5.0.3 - aioseo.com -->\n\t<meta name=\"description\" content=\"MR Imaging of Murine OsteosarcomasWe use magnetic resonance imaging (MRI) to investigate osteosarcomas in mouse legs. 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